What is HHBS?

Posted February 24, 2018

What is HHBS typically used for?

calcium indicator

membrane potential

Answer

Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation in ion channel assays such as calcium channel assays and membrane potential assays.

Additional resources

HHBS Recipe and Preparation Tool

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