What happens during the elution phase in affinity chromatography?

During the elution phase in affinity chromatography, different components elute at different times. Components with the lowest affinity are the first to elute in this technique.

Affinity chromatography is a technique used to separate a biomolecule of interest, usually a protein or protein complex, from a biochemical mixture. It works by manipulating certain molecular properties and specific binding interactions between the target biomolecule and another substance.