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What are the recommended ranges of input RNA amount in reverse transcription reactions?
Posted March 4, 2022
Answer
A snapshot of the recommended ranges of input RNA amount in reverse transcription reactions:
10 pg–5 μg for Total RNA
10 pg–0.5 μg for Poly(A) RNA
0.01 pg–0.5 μg for Specific RNA
Additional resources
Eleven Golden Rules of Quantitative RT-PCR
Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*