What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?

The main differences between NGS and Sanger sequencing are sequencing volume, sequencing length, and cost.

• Sequencing volume: Sanger sequencing can only sequence one single fragment at a time, whereas NGS can read millions of DNA pieces in the flow cell simultaneously per run.
• Sequencing length: NGS methods usually read fragments 100-200 bases long, while Sanger sequencing can read as long as 700-1000 bases in a single sequence.
• Cost: Because of the high-throughput feature of NGS, it is much more cost-effective than Sanger sequencing when sequencing a large amount of DNA.