Is ethidium bromide visible under UV?
Posted December 1, 2021
Answer
Yes, ethidium bromide is visible under UV. Ethidium bromide (EtBr) is commonly used as a non-radioactive dye to visualize DNA in agarose gel electrophoresis experiments.
Ethidium bromide has UV absorbance maxima at 300 and 360 nm and an emission maximum at 590 nm. It fluoresces a red-orange color under UV light and with increased brightness after binding to dsDNA.
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