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Is ethidium bromide used in RT PCR?
Posted November 30, 2021
Answer
Ethidium bromide can be used in RT PCR. However, because of its highly carcinogenic and mutagenic nature, SYBR Green, which is a safe alternative, is generally used in RT PCR.
Additional resources
Ethidium monoazide does not inhibit RT-PCR amplification of nonviable avian influenza RNA
Q4ever™ Green *1250X DMSO Solution*
6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*