logo
AAT Bioquest

Is PE Cy7 photosensitive?

Posted July 1, 2021


Answer

Yes, PE-Cy7 is highly photosensitive.  

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE) and cyanine dye Cy7. This bright dye is often used as a fluorescence-based indicator in flow cytometry for detecting low-abundance antigens. Its use is immunofluorescence microscopy is limited due to its rapid photobleaching property. The dye degrades rapidly when exposed to photobleaching, higher temperatures and paraformaldehyde fixation.

Additional resources

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

PE-Cy7 Tandem

ReadiUse™ Preactivated PE-iFluor™ 750 Tandem