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AAT Bioquest

How much BSA should I add to PCR?

Posted February 11, 2021


Answer

BSA (bovine serum albumin) is particularly useful to enhance the efficiency or specificity of PCR. The concentration depends on the template, but as a general rule, 0.01 µg/µl to 0.5 µg/ µl BSA (final concentration) can be used. 

Additional resources

Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates

ReadiLink™ BSA Conjugation Kit

Polymerase Chain Reaction (PCR)