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How long can I store the oligonucleotide?
Posted October 19, 2020
Answer
Oligonucleotides are relatively stable molecules and be stored for up to a maximum of 2 years under optimum handling and storage conditions. The exact storage time depends on the storage conditions.
| Storage temperature | Storage condition | Approximate storage time at which Oligonucleotides will remain stable |
| Freezer at -20° | Dry or in water or TE buffer solution | 2 years |
| Refrigerator at 4°C or lower temperature | Dry or in water or TE buffer solution |
1 year |
| Room temperature | Dry or in TE buffer solution | 3-6 months |
| Higher temperatures such as when stored in shipping or storage warehouse under non-temperature controlled conditions | Dry or in TE buffer solution | 1-2 months |
The shelf lives mentioned above are estimates only. Various other factors may affect the stability of Oligonucleotides.
To keep Oligonucleotides stable:
- TE buffer is the best option for dissolving Oligonucleotides at all temperatures.
- Water that is used to create the storage solution should also be at neutral pH to prevent depurination. Laboratory-grade water may be slightly acidic, which slowly damages DNA over time.
- Oligonucleotides should never be stored in water at temperatures above 4°C.
Additional resources
In-silico Design of DNA Oligonucleotides: Challenges and Approaches