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AAT Bioquest

How does the autophagy marker in your Cell Meter™ Autophagy Assay Kit (Cat#23000) work?

Posted June 13, 2019


What makes this kit different from your Cell Meter Autophagy Fluorescence Imaging Kit (Cat#23001)?

Answer

The autophagy marker preferentially accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids.

The Autophagy Super Blue maker available in Cat#23001 is an enhanced autophagy probe that generates a stronger more intense fluorescence signal and higher signal-to-background ratio. It’s improved sensitivity for staining autophagosomes versus cell lysosomes greatly reduces background interference.

Additional resources

Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*

Cell Meter™ Autophagy Fluorescence Imaging Kit