How does Immunoassay Test Work?

Immunoassay tests aim at measuring the presence and the concentration of an analyte in blood or other fluids. A sample is applied at one end of the strip, the sample pad, which initiates action due to salts, thus making the reaction mutual with the detection system. After the sample pad has ensured the presence of the target analyte, the conjugate release pad is designed to absorb these treated samples. The detection zone is the final development. If the antibody being tested for is present in the sample, it will react with or bind to the antigen in the test system. A color change occurs. The observed color symbolizes the amount of targeted molecule present in the test solution and will be detected as positive. If there is no significant reaction, the sample tests negative.