How does CRISPR-Cas9 edit genes?

Posted June 22, 2020

Answer

CRISPR-Cas9 cuts genes by creating a double-strand breaks (DSB) in the DNA. Then innate DNA repair mechanisms are automatically triggered within the cell, which can either leave the sequences deleted or insert specific sequences of interest, resulting precise gene editing.

Additional resources

Helixyte™ Green *10,000X Aqueous PCR Solution*

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Doudna, J. A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096.

Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.