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AAT Bioquest

How do you prepare a stock solution of 2,3-Diaminonapthalene?

Posted November 30, 2018


What is the recommended concentration to use when staining cells?

Answer

The recommended concentration for a 2, 3-Diaminonapthalene (15221) stock solution is ~200 mM in DMSO. In this example, we will prepare a 2 mL stock solution of 2, 3-Diaminonapthalene at a concentration of 200 mM.

  1. Prepare 1 mL of DMSO in a suitable container.
  2. Slowly add 63.28 mg of 2, 3-Diaminonapthalene to solution with mixing.
  3. Add DMSO until volume is 2 mL.

When staining cells, the recommended working solution concentration of 2, 3-Diaminonapthalene is ~ 200 μM. After staining incubate your cells for 60 minutes.

Additional resources

2,3-Diaminonaphthalene Product Information Sheet

2,3-Diaminonaphthalene Product Page