How do you evaluate the transfection efficiency?

Posted May 18, 2020

Answer

Transfection efficiency is the percentage of cells that are transfected compared to the entire population. It usually can be evaluated by measuring the expression of reporter genes on the transfection plasmids, such as GFP.

Additional resources

Transfectamine™ 5000 Transfection Reagent

Sandbichler, A. M., Aschberger, T., & Pelster, B. (2013). A Method to Evaluate the Efficiency of Transfection Reagents in an Adherent Zebrafish Cell Line. BioResearch Open Access, 2(1), 20–27. doi:10.1089/biores.2012.0287

Transfection Reagents