How do I stain T cells with CytoTell™ Blue?

1. How much DMSO stock solution do I add to each vial of CytoTell™ Blue (Cat# 22252)?

Add 500uL DMSO/vial

2. How long do I stain the cells before analyzing with flow cytometer?

Incubate cells with a dye solution at room temperature or 37 °C for 30 min, protected from light.

3. How do I stain the cells?

Prepare a 1X dye working solution by diluting the 500X DMSO stock solution at 1 to 500 in Hanks and 20 mM Hepes buffer (HHBS) or the buffer of your choice.