How do I prepare/lyse my cell samples for Amplite® Fluorimetric NAD/NADH Ratio Assay Kit Red Fluorescence?

Posted September 19, 2018

nicotinamide adenine dinucleotide

Answer

Lysis buffer is provided (Component G) . You can use our sample preparation guide outlined in our Cell Sample Preparation Protocol.

Additional resources

Amplite™ Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*

Cell Sample Preparation

Related questions

Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used for plant cells? for bacterial cells? for mammalian cells? for tissue samples?

Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?

Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings?

My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?

Why is the sum of my NAD and NADH extraction readings greater than my Total NAD/NADH reading?