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AAT Bioquest

How do I measure the concentration of zinc ions?

Posted June 13, 2019


I’d like to quantitate the concentration of zinc ions in both biological and environmental samples; e.g. cell culture, plasma, water, etc. Do you provide any products for this purpose?

Answer

Yes, we offer two zinc ion quantitation kits: Amplite Fluorimetric Zinc Ion Quantitation Kit and Amplite Colorimetric Zinc Ion Quantitation Kit. Both kits provide a convenient and robust method for detecting zinc ion concentration in different types of biological samples; e.g. serum, plasma and urine. Our fluorimetric kit utilizes Metal Fluor Zn 520 (Ex/Em = 485/525 nm). Upon binding to zinc, Metal Fluor Zn 520 exhibits a significant fluorescence enhancement, greater than 200-300 folds. Our colorimetric kit utilizes Zn-620. In a zinc-free solution, the absorbance of Zn-620is at 480 nm. When bound to zinc, Zn-620 enhances its absorption to 620 nm. Both probes have high Zn2+-specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+.

Additional resources

Protocol for Amplite™ Fluorimetric Zinc Ion Quantitation Kit

Amplite™ Fluorimetric Zinc Ion Quantitation Kit

Amplite™ Colorimetric Zinc Ion Quantitation Kit

Metal Fluor™ Zn-520, AM