How do I determine transfection efficiency using a flow cytometer?

Using flow cytometry, determining the success of a particular transfection procedure is quite simple:

• Use an untransfected control and subtract the baseline intensity data from your experimental results

• Signal intensity graphs are a simple visual aid to compare the response of the cell line to different transfection reagents

Manual analysis is likely unnecessary; most instruments have software with FACS analysis programs for quick results.