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AAT Bioquest

Does Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit support multiplex assays for detection of mitochondrial superoxide?

Posted June 13, 2019


Answer

Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit (Cat# 16060) uses a unique superoxide indicator MitoROS™ 520 to quantify superoxide level in live cells. MitoROS™ 520 is live-cell permeant and can rapidly and selectively target superoxide in mitochondria. It generates green fluorescence when it reacts with superoxide, and can be easily read at Ex/Em = 490/530 nm. The Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit provides a sensitive, one-step fluorimetric assay to detect mitochondrial superoxide in live cells with one hour incubation. This kit can be used for flow cytometry and fluorescence microscopy applications.

Additional resources

Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit

MitoROS™ 580 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*