Can proteases be degraded by themselves?
Posted July 22, 2020
Answer
Yes, they can. Proteases can be cleaved by other protease molecules, either another unit of the same protease or a different protease, as long as they contain a specific cleavage site for them to recognize. This process is called autolysis, which acts as a method of regulating protease activity. Some proteases are less active after autolysis (e.g. TEV protease), while others are more active (e.g. trypsinogen).
Additional resources
Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.