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AAT Bioquest

Can I use your Cell Meter Apoptotic and Necrotic Multiplexing Detection Kits with a fluorescence microplate reader?

Posted April 19, 2018


If yes, do you have a recommended protocol?

Answer

No, our Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kits are not intended for use with a microplate reader. These kits are designed to be used with a fluorescence microscope or flow cytometer.

These kits employ fluorescent probes which require washing steps prior to imaging. Therefore, it is difficult to maintain the same cell number in control wells and in wells containing apoptotic cells because apoptotic cells can be easily washed away.

Additional resources

Product page for Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I

Product page for Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II

Product page for Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit III