RPMI 1640 Medium Recipe

Component ▲ ▼	Molecular Weight ▲ ▼	Type ▲ ▼	Concentration (mM) ▲ ▼	Total Mass (mg) ▲ ▼
Biotin	244.0	Vitamin	0.00081967	0.2
Calcium nitrate (Ca(NO3)2-4H2O)	236.0	Inorganic Salt	0.42373	100
Choline chloride	140.0	Vitamin	0.021429	3
D-Calcium pantothenate	477.0	Vitamin	0.00052411	0.25
D-Glucose (Dextrose)	180.16	Other	11.1012	2000
Folic acid	441.0	Vitamin	0.0022676	1
Glutathione (reduced)	307.0	Other	0.0032573	1
Glycine	75.07	Amino Acid	0.13321	10
i-Inositol	180.0	Vitamin	0.19444	35
L-Arginine	174.20	Amino Acid	1.1481	200
L-Asparagine	132.0	Amino Acid	0.37879	50
L-Aspartic acid	133.10	Amino Acid	0.15026	20
L-Cystine 2HCl	313.0	Amino Acid	0.20767	65
L-Glutamic acid	147.0	Amino Acid	0.13605	20
L-Glutamine	146.0	Amino Acid	2.0548	300
L-Histidine	155.15	Amino Acid	0.096681	15
L-Hydroxyproline	131.0	Amino Acid	0.15267	20
L-Isoleucine	131.17	Amino Acid	0.38118	50
L-Leucine	131.17	Amino Acid	0.38118	50
L-Lysine hydrochloride	183.0	Amino Acid	0.21858	40
L-Methionine	149.21	Amino Acid	0.10053	15
L-Phenylalanine	165.19	Amino Acid	0.090805	15
L-Proline	115.0	Amino Acid	0.17391	20
L-Serine	105.0	Amino Acid	0.28571	30
L-Threonine	119.12	Amino Acid	0.1679	20
L-Tryptophan	204.23	Amino Acid	0.024482	5
L-Tyrosine disodium salt dihydrate	261.0	Amino Acid	0.11111	29
L-Valine	117.15	Amino Acid	0.17072	20
Magnesium Sulfate (MgSO₄) (anhyd.)	120.0	Inorganic Salt	0.407	48.84
Niacinamide	122.0	Vitamin	0.0081967	1
Para-aminobenzoic acid	137.0	Vitamin	0.0072993	1
Phenol Red	354.38	Other	0.014109	5
Potassium Chloride (KCl)	75.0	Inorganic Salt	5.3333	400
Pyridoxine hydrochloride	206.0	Vitamin	0.0048544	1
Riboflavin	376.0	Vitamin	0.00053191	0.2
Sodium Bicarbonate (NaHCO₃)	84.0	Inorganic Salt	23.8095	2000
Sodium Chloride (NaCl)	58.0	Inorganic Salt	103.4483	6000
Sodium phosphate dibasic (Na2HPO4) (anhyd.)	142.0	Inorganic Salt	5.6338	800
Thiamine hydrochloride	337.0	Vitamin	0.0029674	1
Vitamin B12	1355.0	Vitamin	0.00000369	0.005

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MLA	"Quest Calculate™ RPMI 1640 Medium Recipe." AAT Bioquest, Inc., 23 Apr. 2025, https://www.aatbio.com/resources/cell-media-formulations/rpmi-1640-medium-formulation-composition-recipe-and-preparation.
APA	AAT Bioquest, Inc. (2025, April 23). Quest Calculate™ RPMI 1640 Medium Recipe. AAT Bioquest. https://www.aatbio.com/resources/cell-media-formulations/rpmi-1640-medium-formulation-composition-recipe-and-preparation.
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RPMI 1640 Medium Recipe

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