DMEM/F-12, powder, HEPES Recipe

Component ▲ ▼	Molecular Weight ▲ ▼	Type ▲ ▼	Concentration (mM) ▲ ▼	Total Mass (mg) ▲ ▼
Biotin	244.0	Vitamin	0.000014344	0.0035
Calcium Chloride (CaCl₂) (anhyd.)	111.0	Inorganic Salt	1.0505	116.6
Choline chloride	140.0	Vitamin	0.064143	8.98
Cupric sulfate (CuSO4-5H2O)	250.0	Inorganic Salt	0.0000052	0.0013
D-Calcium pantothenate	477.0	Vitamin	0.004696	2.24
D-Glucose (Dextrose)	180.16	Other	17.49	3151
Ferric Nitrate (Fe(NO₃)₃-9H₂O)	404.0	Inorganic Salt	0.00012376	0.05
Ferric sulfate (FeSO4-7H2O)	278.0	Inorganic Salt	0.0015	0.417
Folic acid	441.0	Vitamin	0.0060091	2.65
Glycine	75.07	Amino Acid	0.24977	18.75
Hepes	238.30	Other	15	3574.5
Hypoxanthine Na	159.0	Other	0.015031	2.39
i-Inositol	180.0	Vitamin	0.07	12.6
L-Alanine	89.0	Amino Acid	0.05	4.45
L-Arginine hydrochloride	211.0	Amino Acid	0.69905	147.5
L-Asparagine-H₂O	150.0	Amino Acid	0.05	7.5
L-Aspartic acid	133.10	Amino Acid	0.049962	6.65
L-Cysteine hydrochloride-H₂O	176.0	Amino Acid	0.099773	17.56
L-Cystine 2HCl	313.0	Amino Acid	0.099968	31.29
L-Glutamic acid	147.0	Amino Acid	0.05	7.35
L-Glutamine	146.0	Amino Acid	2.5	365
L-Histidine hydrochloride-H₂O	210.0	Amino Acid	0.1499	31.48
L-Isoleucine	131.17	Amino Acid	0.41526	54.47
L-Leucine	131.17	Amino Acid	0.45018	59.05
L-Lysine hydrochloride	183.0	Amino Acid	0.49863	91.25
L-Methionine	149.21	Amino Acid	0.11554	17.24
L-Phenylalanine	165.19	Amino Acid	0.21478	35.48
L-Proline	115.0	Amino Acid	0.15	17.25
L-Serine	105.0	Amino Acid	0.25	26.25
L-Threonine	119.12	Amino Acid	0.44871	53.45
L-Tryptophan	204.23	Amino Acid	0.044166	9.02
L-Tyrosine disodium salt dihydrate	261.0	Amino Acid	0.21375	55.79
L-Valine	117.15	Amino Acid	0.45113	52.85
Linoleic acid	280.0	Other	0.00015	0.042
Lipoic acid	206.3	Other	0.00050897	0.105
Magnesium chloride (anhyd.)	95.0	Inorganic Salt	0.30147	28.64
Magnesium Sulfate (MgSO₄) (anhyd.)	120.0	Inorganic Salt	0.407	48.84
Niacinamide	122.0	Vitamin	0.016557	2.02
Phenol Red	354.38	Other	0.022857	8.1
Potassium Chloride (KCl)	75.0	Inorganic Salt	4.1573	311.8
Putrescine 2HCl	161.0	Other	0.00050311	0.081
Pyridoxine hydrochloride	206.0	Vitamin	0.0098592	2.031
Riboflavin	376.0	Vitamin	0.00058245	0.219
Sodium Chloride (NaCl)	58.0	Inorganic Salt	120.6121	6995.5
Sodium phosphate dibasic (Na2HPO4) (anhyd.)	142.0	Inorganic Salt	0.50014	71.02
Sodium Phosphate monobasic (NaH₂PO₄-H₂O)	138.0	Inorganic Salt	0.4529	62.5
Sodium pyruvate	110.0	Other	0.5	55
Thiamine hydrochloride	337.0	Vitamin	0.0064392	2.17
Thymidine	242.0	Deoxyribonucleoside	0.0015083	0.365
Vitamin B12	1355.0	Vitamin	0.00050185	0.68
Zinc sulfate (ZnSO4-7H2O)	288.0	Inorganic Salt	0.0015	0.432

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MLA	"Quest Calculate™ DMEM/F-12, powder, HEPES Recipe." AAT Bioquest, Inc., 24 Nov. 2024, https://www.aatbio.com/resources/cell-media-formulations/dmem-f-12-powder-hepes-formulation-composition-recipe-and-preparation.
APA	AAT Bioquest, Inc. (2024, November 24). Quest Calculate™ DMEM/F-12, powder, HEPES Recipe. AAT Bioquest. https://www.aatbio.com/resources/cell-media-formulations/dmem-f-12-powder-hepes-formulation-composition-recipe-and-preparation.
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DMEM/F-12, powder, HEPES Recipe

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