ReadiLink™ Antibody Labeling Technology

In this issue

Horseradish Peroxidase (HRP)
ReadiLink™ Antibody Labeling Technology
ReadiView™ Biotinylation Technology
TR-FRET Assays
RNA Detection
Fluorescent Calcium Indicators
Calcein-Based Cell Viability Assays
Fluorescence Live Cell Imaging

AssayWise Letters 2016, Vol. 5(1)
cover

PDF

ReadiLink™ iFluor^® Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor^® dyes. The iFluor^® dyes show good reactivity and selectivity with the aliphatic amines of antibodies and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond. iFluor^®-antibody conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit comes with all the essential components for performing the conjugation reaction and for purifying the iFluor^®-antibody conjugates. ReadiLink™ Kits quickly label your antibody of interest in two easy steps with the unique chemistry of ReadiLink™ Dyes. The conjugation kits provide the best method for readily labeling microscale volumes without requiring column purification.

Key Features of ReadiLink™ Kits:

Save time with no purification
Conjugate your antibodies in 2 easy
Available in 10 different fluorophores with excitation wavelengths ranging from UV to infrared.

ReadiLink™ Kit Labeling Principle

Start the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the reaction buffer (pH 7.5-8.5).
Incubate the reaction solution and get a mixture of the desired protein conjugate and unreactive free dye.
Quench the reaction by mixing a non-fluorescent Tide Quencher™ (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-labeled dye complex, which eliminates the background fluorescence interference of the free labeling dye.

Table 1. ReadiLink™ iFluor® Antibody Labeling Assays

Cat No. ▲ ▼	Product Name ▲ ▼	Alternative to ▲ ▼	Ex (nm) ▲ ▼	Em (nm) ▲ ▼	Unit Size ▲ ▼
1299	ReadiLink™ Rapid FITC Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	492	516	2 Labelings
1220	ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	345	442	2 Labelings
1255	ReadiLink™ Rapid iFluor® 488 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	491	514	2 Labelings
1227	ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	559	569	2 Labelings
1230	ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	592	614	2 Labelings
1260	ReadiLink™ Rapid iFluor® 633 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	638	655	2 Labelings
1235	ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	654	674	2 Labelings
1240	ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	682	701	2 Labelings
1245	ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	693	713	2 Labelings
1250	ReadiLink™ Rapid iFluor® 750 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	753	779	2 Labelings
1265	ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit Microscale Optimized for Labeling 50 µg Antibody Per Reaction	Lightning-Link® Dye Labeling Kits	782	811	2 Labelings

Items per page: 1 - 10 of 11

⟨⟩