Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency for longer wavelength dyes (such as fluoresceins, rhodamines, and cyanines) has limited its use in the development of sensitive FRET probes. AAT Bioquest has developed the robust Tide Quencher™acceptor dyes for development of longer wavelength FRET probes. These Tide Quencher™ dark FRET acceptors are optimized to pair with ourbright Tide Fluor™ dyes and the classic fluorophores (such as EDANS, FAM, TAMRA, HEX, JOE, TET, ROX, Cy3, Cy5, and CY7). Like our Tide Fluor™ donor dyes, our Tide Quencher™ acceptor dyes are much more cost-effective with comparable or even better performance for your desired biological applications than the other commercial products on the market.

 

AAT Bioquest offers a full set of Tide Quencher™ dyes that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher^® and QSY^® dyes. They can be readily used to develop various FRET probes for molecular diagnostics, proteomics and other biological applications.

Tide Quencher™ dyes are a series of excellent labeling quenchers that span the full visible spectrum. Tide Quencher™ dyes have improved labeling performance than the classic quencher dyes such as Dabcyl, Black Hole Quencher^® and QSY^® dyes. They are the best affordable dyes for labeling oligos and peptides without comprised performance. Each Tide Quencher™ dye is developed to match the spectral properties of a particular fluorescent donor, e.g., Alexa Fluor^® or other labeling dyes (such as DyLight™ dyes).