Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications

Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications

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• Optimized to label peptides and nucleotides with performance comparable to Alexa Fluor^® Dyes
• Optimized to pair with Tide Quencher™ dark acceptors to maximize FRET efficiency
• pH-insensitive and environment-insensitive fluorescence for developing robust assays
• Higher photostability to improve the quality of fluorescence imaging
• A variety of reactive forms available for conjugations

The sulfonated cyanine dyes have improved fluorescence quantum yield than those of non-sulfonate cyanines. They are commercially available as Cy3^®, Cy5^®, Cy5.5^® and Cy7^®. However, these commercial protein-labeling CyDyes and Alexa Fluor^® dyes are cost-prohibitive for labeling peptides and oligonucleotides that require much larger amount of labeling dyes. The improved and affordable Tide Fluor™ dyes are optimized as building blocks for developing FRET oligonucleotides and peptides. Our Tide Fluor™ dyes have stronger fluorescence and higher photostability than the classic fluorophores (such as fluoresceins, rhodamines and cyanines). They are the best affordable fluorescent dyes for labeling peptides and oligonucleotides without comprised performance.

Chemical Properties of Tide Fluor™ Fluorescent Labeling Dyes

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Tide Fluor™ dyes are a series of excellent fluorescent labeling dyes that span the full visible spectrum. Tide Fluor™ dyes have improved labeling performance than the classic fluorescent labeling dyes such as FITC, TRITC, Texas Red^®, Cy3, Cy5 and Cy7. They are the best affordable fluorescent dyes (alternative to Alexa Fluor^® dyes) for labeling oligos and peptides without comprised performance. Each Tide Fluor™ dye is developed to match the spectral properties of a particular Alexa Fluor^® or other labeling dyes (such as DyLight™ dyes).

1. * The molecular weight of the desired conjugate = the molecular weight of the free unlabeled molecule + the value listed in the table.

Spectral Properties of Tide Fluor™ Fluorescent Labeling Dyes

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1. Extinction Coefficient at their maximum absorption wavelength
2. FQY = fluorescence quantum yield in aqueous buffer (pH 7.2)
3. CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling)
4. CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling)
5. Fluorescence intensity is significantly increased upon coupled to proteins or long peptides.