Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications
Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications
- Optimized to label peptides and nucleotides with performance comparable to Alexa Fluor® Dyes
- Optimized to pair with Tide Quencher™ dark acceptors to maximize FRET efficiency
- pH-insensitive and environment-insensitive fluorescence for developing robust assays
- Higher photostability to improve the quality of fluorescence imaging
- A variety of reactive forms available for conjugations
Table 1. Tide Fluor™ Dyes for Developing FRET Probes
Tide Fluor™ Donor ▲ ▼ | Ex (nm) ▲ ▼ | Em (nm) ▲ ▼ | Features and Benefits ▲ ▼ | Ordering Information ▲ ▼ |
Tide Fluor™ 1 (TF1) | 345 | 442 |
|
Cat# 2236 (TF1 azide, click chemistry)
Cat# 2237 (TF1 alkyne, click chemistry) Cat# 2238 (TF1 acid) Cat# 2239 (TF1 amine) Cat# 2242 (TF1 maleimide, SH-reactive) Cat# 2244 (TF1 SE, NH2-reactive) |
Tide Fluor™ 2 (TF2) | 500 | 527 |
|
Cat# 2245 (TF1 acid)
Cat# 2246 (TF2 amine) Cat# 2247 (TF2 maleimide SH-reactive) Cat# 2248 (TF2 SE, NH2-reactive) Cat# 2252 (TF2 azide, click chemistry) Cat# 2253 (TF2 alkyne, click chemistry) |
Tide Fluor™ 3 (TF3) | 555 | 584 |
|
Cat# 2254 (TF3 azide, click chemistry)
Cat# 2255 (TF3 alkyne, click chemistry) Cat# 2268 (TF3 acid) Cat# 2269 (TF3 amine) Cat# 2270 (TF3 maleimide, SH-reactive) Cat# 2271 (TF3 SE, NH2-reactive) |
Tide Fluor™ 3WS (TF3WS) | 555 | 565 |
|
Cat# 2345 (TF3WS acid)
Cat# 2346 (TF3WS SE, NH2-reactive) |
Tide Fluor™ 4 (TF4) | 590 | 618 |
|
Cat# 2285 (TF4 acid)
Cat# 2286 (TF4 amine) Cat# 2287 (TF4 maleimide, SH-reactive) Cat# 2289 (TF4 SE, NH2-reactive) Cat# 2300 (TF4 azide, click chemistry) Cat# 2301 (TF4 alkyne, click chemistry) |
Tide Fluor™ 5WS (TF5WS) | 649 | 664 |
|
Cat# 2275 (TF5WS azide, click chemistry)
Cat# 2276 (TF5WS alkyne, click chemistry) Cat# 2278 (TF5WS, acid) Cat# 2279 (TF5WS amine) Cat# 2280 (TF5WS maleimide, SH-reactive) Cat# 2281 (TF5WS SE, NH2-reactive) |
Tide Fluor™ 6WS (TF6WS) | 676 | 695 |
|
Cat# 2291 (TF6WS acid)
Cat# 2292 (TF6WS amine) Cat# 2293 (TF6WS maleimide, SH-reactive) Cat# 2294 (TF6WS SE, NH2-reactive) Cat# 2302 (TF6WS azide, click chemistry) Cat# 2303 (TF6WS alkyne, click chemistry) |
Tide Fluor™ 7WS (TF7WS) | 749 | 775 |
|
Cat# 2304 (TF7WS azide, click chemistry)
Cat# 2305 (TF7WS alkyne, click chemistry) Cat# 2330 (TF7WS acid) Cat# 2331 (TF7WS amine) Cat# 2332 (TF7WS maleimide, SH-reactive) Cat# 2333 (TF7WS SE, NH2-reactive) |
Tide Fluor™ 8WS (TF8WS) | 775 | 807 |
|
Cat# 2306 (TF8WS azide, click chemistry)
Cat# 2307 (TF8WS alkyne, click chemistry) Cat# 2335 (TF8WS acid) Cat# 2336 (TF8WS amine) Cat# 2337 (TF8WS maleimide, SH-reactive) Cat# 2338 (TF8WS SE, NH2-reactive) |
Chemical Properties of Tide Fluor™ Fluorescent Labeling Dyes
Tide Fluor™ dyes are a series of excellent fluorescent labeling dyes that span the full visible spectrum. Tide Fluor™ dyes have improved labeling performance than the classic fluorescent labeling dyes such as FITC, TRITC, Texas Red®, Cy3, Cy5 and Cy7. They are the best affordable fluorescent dyes (alternative to Alexa Fluor® dyes) for labeling oligos and peptides without comprised performance. Each Tide Fluor™ dye is developed to match the spectral properties of a particular Alexa Fluor® or other labeling dyes (such as DyLight™ dyes).
Table 2. Chemical Properties of Tide Fluor™ Fluorescent Labeling Dyes
Cat No. ▲ ▼ | Labeling Dye ▲ ▼ | Reactivity ▲ ▼ | Adduct MW Calculation* ▲ ▼ |
2236 | Tide Fluor™ 1 azide [TF1 azide] | Alkyne | + 301 |
2237 | Tide Fluor™ 1 alkyne [TF1 alkyne] | Azide | + 270 |
2238 | Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS* | NH2 and OH | + 215 |
2239 | Tide Fluor™ 1 amine [TF1 amine] *Superior replacement for EDANS* | CO2H | + 257 |
2242 | Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* | SH | + 355 |
2244 | Tide Fluor™ 1 succinimidyl ester [TF1 SE] | Aliphatic amine | + 215 |
2245 | Tide Fluor™ 2 acid [TF2 acid] *Superior replacement for fluorescein* | NH2 and OH | + 469 |
2246 | Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein* | CO2H | + 398 |
2247 | Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | SH | + 680 |
2248 | Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein* | Aliphatic amine | + 469 |
- * The molecular weight of the desired conjugate = the molecular weight of the free unlabeled molecule + the value listed in the table.
Spectral Properties of Tide Fluor™ Fluorescent Labeling Dyes
Table 3. Tide Fluor™ Dyes Spectral Properties
Labeling Dye ▲ ▼ | Abs (mn) ▲ ▼ | Em (nm) ▲ ▼ | ε¹ ▲ ▼ | Φ² ▲ ▼ | CF at 260 nm³ ▲ ▼ | CF at 280 nm⁴ ▲ ▼ |
Tide Fluor™ 1 | 345 | 442 | 20000 | 0.95 | 0.246 | 0.187 |
Tide Fluor™ 2WS | 491 | 516 | 75000 | 0.9 | 0.211 | 0.091 |
Tide Fluor™ 2 | 500 | 527 | 75000 | 0.9 | 0.288 | 0.201 |
Tide Fluor™ 3WS | 555 | 565 | 150000 | 0.105 | 0.079 | 0.079 |
Tide Fluor™ 3 | 555 | 584 | 85000 | 0.85 | 0.331 | 0.201 |
Tide Fluor™ 4 | 590 | 618 | 90000 | 0.91 | 0.489 | 0.436 |
Tide Fluor™ 5WS | 649 | 664 | 250000 | 0.25 | 0.023 | 0.027 |
Tide Fluor™ 6WS | 676 | 695 | 220000 | 0.18 | 0.111 | 0.009 |
Tide Fluor™ 7WS | 749 | 775 | 275000 | 0.12 | 0.009 | 0.049 |
Tide Fluor™ 8WS | 775 | 807 | 250000 | 0.08 | 0.103 | 0.109 |
- Extinction Coefficient at their maximum absorption wavelength
- FQY = fluorescence quantum yield in aqueous buffer (pH 7.2)
- CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling)
- CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling)
- Fluorescence intensity is significantly increased upon coupled to proteins or long peptides.
Original created on January 3, 2020, last updated on October 27, 2022
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