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Z-Gly-Pro-AMC *CAS 68542-93-8*

Z-Gly-Pro-AMC is a fluorogenic substrate for the determination of post-proline cleaving enzyme (prolyl endopeptidase). This substrate is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (AMC).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-Gly-Pro-AMC *CAS 68542-93-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM215.754 µL1.079 mL2.158 mL10.788 mL21.575 mL
5 mM43.151 µL215.754 µL431.509 µL2.158 mL4.315 mL
10 mM21.575 µL107.877 µL215.754 µL1.079 mL2.158 mL

Molarity calculator

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Spectrum

Citations

View all 1 citations: Citation Explorer
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice
Authors: Xiao, Fei and Jiang, Haizhou and Li, Zi and Jiang, Xiaoxue and Chen, Shanghai and Niu, Yuguo and Yin, Hanrui and Shu, Yousheng and Peng, Bo and Lu, Wei and others,
Journal: Nature Communications (2023): 2523

References

View all 2 references: Citation Explorer
Antiplasmin-cleaving enzyme is a soluble form of fibroblast activation protein
Authors: Lee KN, Jackson KW, Christiansen VJ, Lee CS, Chun JG, McKee PA.
Journal: Blood (2006): 1397
Substrate-dependent, non-hyperbolic kinetics of pig brain prolyl oligopeptidase and its tight binding inhibition by JTP-4819
Authors: Venalainen JI, Juvonen RO, Forsberg MM, Garcia-Horsman A, Poso A, Wallen EA, Gynther J, Mannisto PT.
Journal: Biochem Pharmacol (2002): 463
Page updated on November 21, 2024

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Physical properties

Molecular weight

463.49

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

68542-93-8
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