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AAT Bioquest

XFD633 acid

Product key features

  • Ex/Em: 632/650 nm
  • Extinction Coefficient: 159,000 cm-1M-1
  • Non-reactive Acid: Serves as reference standard, enables diverse conjugations, most cost-effective format for imaging and synthesis
  • Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 633
  • Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

Product description

XFD633, manufactured by AAT Bioquest, is a bright red-fluorescent dye structurally identical to Alexa Fluor™ 633 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD633 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

XFD633 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD633 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Page updated on March 11, 2025

Ordering information

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Catalog Number70070
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.55

Extinction coefficient (cm -1 M -1)

159,000

Excitation (nm)

632

Emission (nm)

650

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501