XFD610 PEG4 DBCO

• Ex/Em: 611/629 nm
• Extinction coefficient: 144,000 cm^-1M^-1
• Reactive Group: DBCO
• Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via SPAAC
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-10) with exceptional photostability
• Hydrophilic PEG4 Spacer: Enhances solubility and minimizes steric hindrance for enhanced biocompatibility

XFD610, manufactured by AAT Bioquest, is a red-fluorescent dye that is structurally identical to Alexa Fluor™ 610 (ThermoFisher). The dye exhibits high photostability and strong fluorescence, making it well-suited for advanced imaging applications and flow cytometry. Its water solubility and pH-independent fluorescence over a broad range (pH 4–10) ensure consistent performance under diverse experimental conditions. XFD610 enables reproducible labeling with high signal intensity, providing reliable results in fluorescence-based assays. Additionally, it serves as a robust alternative to Texas Red™, offering improved optical properties for experiments requiring enhanced fluorescence output.

The DBCO derivative of XFD610 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD610 PEG4 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.