Wheat Germ Agglutinin (WGA)

mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate

Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The mFluor™ Violet 500 labeled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 501 nm. Notably, the mFluor™ Violet 500 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis of various scientific investigations.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25452	1 mg	Price

References

View all 50 references: Citation Explorer

Pig sperm membrane microdomains contain a highly glycosylated 15-25-kDa wheat germ agglutinin-binding protein.

Authors:

Kasekarn, Waraporn and Kanazawa, Takeru and Hori, Kazuki and Tsuchiyama, Tomoyuki and Lian, Xue and Garénaux, Estelle and Kongmanas, Kessiri and Tanphaichitr, Nongnuj and Yasue, Hiroshi and Sato, Chihiro and Kitajima, Ken

Journal:

Biochemical and biophysical research communications (2012): 356-62

Measurement of wheat germ agglutinin binding with a fluorescence microscope.

Authors:

Model, Michael A and Reese, Jennifer L and Fraizer, Gail C

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 874-81

Characterization of the wheat germ agglutinin binding to self-assembled monolayers of neoglycoconjugates by AFM and SPR.

Authors:

Lienemann, Michael and Paananen, Arja and Boer, Harry and de la Fuente, Jesús M and García, Isabel and Penadés, Soledad and Koivula, Anu

Journal:

Glycobiology (2009): 633-43

Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid.

Authors:

Fodero, L R and Sáez-Valero, J and Barquero, M S and Marcos, A and McLean, C A and Small, D H

Journal:

Journal of neurochemistry (2001): 1022-6

Distribution of concanavalin A and wheat germ agglutinin binding sites in the rat peripheral nerve fibres revealed by lectin/glycoprotein-gold histochemistry.

Authors:

Dolapchieva, S

Journal:

The Histochemical journal (1996): 7-12

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	412
Correction factor (260 nm)	0.769
Correction factor (280 nm)	0.365
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	410
Emission (nm)	501
Quantum yield	0.81 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	410 nm
Emission	501 nm
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 8, 2024