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TRITC–dextran conjugate (average MW = ~20K)
TRITC-labeled dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. It is also used in microcirculation and cell permeability research utilizing microfluorimetry, and possible in perfusion studies in animals. TRITC-dextran has also been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to TRITC-dextran. Small TRITC-dextrans are often used to measure processes such as endocytosis and cell junction permeability. Rhodamine-dextran, 20 kDa, may be used to study cell processes (such as endocytosis and osmoporation), endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability and drug release from structures such as hydrogens and collagen minirods.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) | Correction Factor (260 nm) |
| FITC–dextran conjugate (average MW = ~20K) | 491 | 516 | 73000 | 0.35 | - |
| TRITC–dextran conjugate (average MW = ~70K) | 544 | 570 | 100000 | 0.34 | 0.27 |
| TRITC–dextran conjugate (average MW = ~150K) | 544 | 570 | 100000 | 0.34 | 0.27 |
Citations
View all 7 citations: Citation Explorer
SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control
Authors: Shen, W. B., Vaccaro, D. E., Fishman, P. S., Groman, E. V., Yarowsky, P.
Journal: Contrast Media Mol Imaging (2016): 222-8
Authors: Shen, W. B., Vaccaro, D. E., Fishman, P. S., Groman, E. V., Yarowsky, P.
Journal: Contrast Media Mol Imaging (2016): 222-8
In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran
Authors: Volkmann, K., Koster, R. W.
Journal: CSH Protoc (2007): pdb prot4832
Authors: Volkmann, K., Koster, R. W.
Journal: CSH Protoc (2007): pdb prot4832
Tracing of axonal connectivities in a combined slice preparation of rat brains--a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala
Authors: von Bohlen und Halbach, O., Albrecht, D.
Journal: J Neurosci Methods (1998): 169-75
Authors: von Bohlen und Halbach, O., Albrecht, D.
Journal: J Neurosci Methods (1998): 169-75
Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine
Authors: Dolleman-Van der Weel, M. J., Wouterlood, F. G., Witter, M. P.
Journal: J Neurosci Methods (1994): 9-21
Authors: Dolleman-Van der Weel, M. J., Wouterlood, F. G., Witter, M. P.
Journal: J Neurosci Methods (1994): 9-21
Tracing of axonal connections by rhodamine-dextran-amine in the rat hippocampal-entorhinal cortex slice preparation
Authors: Boulton, C. L., von Haebler, D., Heinemann, U.
Journal: Hippocampus (1992): 99-106
Authors: Boulton, C. L., von Haebler, D., Heinemann, U.
Journal: Hippocampus (1992): 99-106
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