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TMA-DPH *CAS#: 115534-33-3*
TMA-DPH is a fluorescent probe for membrane fluidity measurements. It is especially useful in the study of monolayer dynamics of lipoproteins and similar analogous systems. DPH and its derivatives (such as TMA-DPH) are cylinder-shaped and have fluorescence emission transition dipoles that are basically aligned parallel to their long molecular axis. As such, they are very sensitive to reorientation resulting from interactions with surrounding lipids. They are widely used in fluorescence polarization studies of rotational motion.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TMA-DPH *CAS#: 115534-33-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 216.628 µL | 1.083 mL | 2.166 mL | 10.831 mL | 21.663 mL |
| 5 mM | 43.326 µL | 216.628 µL | 433.257 µL | 2.166 mL | 4.333 mL |
| 10 mM | 21.663 µL | 108.314 µL | 216.628 µL | 1.083 mL | 2.166 mL |
Molarity calculator
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Citations
View all 10 citations: Citation Explorer
Toxicity of hexagonal boron nitride nanosheets to freshwater algae: Phospholipid membrane damage and carbon assimilation inhibition
Authors: Zou, Wei and Huo, Yuhan and Zhang, Xingli and Jin, Caixia and Li, Xiaokang and Cao, Zhiguo
Journal: Journal of Hazardous Materials (2023): 133204
Authors: Zou, Wei and Huo, Yuhan and Zhang, Xingli and Jin, Caixia and Li, Xiaokang and Cao, Zhiguo
Journal: Journal of Hazardous Materials (2023): 133204
Cis-and trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species
Authors: Wang, Chaogang and Li, Ao and Cong, Rihao and Qi, Haigang and Wang, Wei and Zhang, Guofan and Li, Li
Journal: Molecular Biology and Evolution (2023)
Authors: Wang, Chaogang and Li, Ao and Cong, Rihao and Qi, Haigang and Wang, Wei and Zhang, Guofan and Li, Li
Journal: Molecular Biology and Evolution (2023)
Size-dependent effects of polystyrene microplastics on cytotoxicity and efflux pump inhibition in human Caco-2 cells
Authors: Wu, Bing and Wu, Xiaomei and Liu, Su and Wang, Zhizhi and Chen, Ling
Journal: Chemosphere (2019)
Authors: Wu, Bing and Wu, Xiaomei and Liu, Su and Wang, Zhizhi and Chen, Ling
Journal: Chemosphere (2019)
Comparison of cytotoxicity and membrane efflux pump inhibition in HepG2 cells induced by single-walled carbon nanotubes with different length and functional groups
Authors: Shen, Zhuoyan and Wu, Jialu and Yu, Yue and Liu, Su and Jiang, Wei and Nurmamat, Habiba and Wu, Bing
Journal: Scientific Reports (2019): 7557
Authors: Shen, Zhuoyan and Wu, Jialu and Yu, Yue and Liu, Su and Jiang, Wei and Nurmamat, Habiba and Wu, Bing
Journal: Scientific Reports (2019): 7557
Interaction of Hsp90 with phospholipid model membranes
Authors: Zhang, Muhan and Wang, Daoying and Li, Pengpeng and Sun, Chong and Xu, Rong and Geng, Zhiming and Xu, Weimin and Dai, Zhaoqi
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2018): 611--616
Authors: Zhang, Muhan and Wang, Daoying and Li, Pengpeng and Sun, Chong and Xu, Rong and Geng, Zhiming and Xu, Weimin and Dai, Zhaoqi
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2018): 611--616
References
View all 16 references: Citation Explorer
Fluorescent probes DPH, TMA-DPH and C17-HC induce erythrocyte exovesiculation
Authors: Saldanha C, Santos NC, Martins-Silva J.
Journal: J Membr Biol (2002): 75
Authors: Saldanha C, Santos NC, Martins-Silva J.
Journal: J Membr Biol (2002): 75
Fluorescent lipid probes 12-AS and TMA-DPH report on selective, purinergically induced fluidity changes in plasma membranes of lymphoid cells
Authors: Matko J, Nagy P.
Journal: J Photochem Photobiol B (1997): 120
Authors: Matko J, Nagy P.
Journal: J Photochem Photobiol B (1997): 120
Plasma membrane fluidity of keratinocytes of normal and psoriatic skin: a study using fluorescence anisotropy of trimethylammoniumdiphenylhexatriene (TMA-DPH)
Authors: Simonetti O, Ferretti G, Offidani AM, Gervasi P, Curatola G, Bossi G.
Journal: Arch Dermatol Res (1996): 51
Authors: Simonetti O, Ferretti G, Offidani AM, Gervasi P, Curatola G, Bossi G.
Journal: Arch Dermatol Res (1996): 51
Enzymically generated electron-excited species increase the membrane order parameter without changing the rotational relaxation time of TMA-DPH
Authors: Obsil T, Amler E, Kotyk A, Pavlicek Z.
Journal: Gen Physiol Biophys (1995): 393
Authors: Obsil T, Amler E, Kotyk A, Pavlicek Z.
Journal: Gen Physiol Biophys (1995): 393
A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane
Authors: Illinger D, Duportail G, Mely Y, Poirel-Morales N, Gerard D, Kuhry JG.
Journal: Biochim Biophys Acta (1995): 58
Authors: Illinger D, Duportail G, Mely Y, Poirel-Morales N, Gerard D, Kuhry JG.
Journal: Biochim Biophys Acta (1995): 58
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