Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]Superior replacement for Cy5

Tide Fluor™ 5WS (TF5WS) family has the spectral properties essentially identical to those of Cy5. Compared to Cy5 probes TF5WS family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy5. TF5WS-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy5. In pairing with our Tide Quencher™ 5 (TQ5), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]*Superior replacement for Cy5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.206 µL	476.032 µL	952.064 µL	4.76 mL	9.521 mL
5 mM	19.041 µL	95.206 µL	190.413 µL	952.064 µL	1.904 mL
10 mM	9.521 µL	47.603 µL	95.206 µL	476.032 µL	952.064 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
Tide Fluor™ 1 succinimidyl ester [TF1 SE]Superior replacement for EDANS	341	447	20000	0.91¹	0.187
Tide Fluor™ 2, succinimidyl ester [TF2 SE]Superior replacement for fluorescein	503	525	75000	0.9¹	0.09
Tide Fluor™ 3 succinimidyl ester [TF3 SE]Superior replacement for Cy3	553	578	75000¹	0.1¹	0.179
Tide Fluor™ 4, succinimidyl ester [TF4 SE]Superior replacement for ROX and Texas Red	577	602	90000	0.77¹	0.436
Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]Superior replacement for Cy5.5	682	701	220000	0.27¹	0.101
Tide Fluor™ 7WS, succinimidyl ester [TF7WS SE]Superior replacement for Cy7	756	780	275000	0.3¹	0.049
Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]Near Infrared Emission	785	801	250000	0.21¹	0.109
Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] Superior replacement for Cy3	550	563	150000	0.15¹	0.079
Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] Superior replacement for FITC	491	516	75000	0.9¹	0.11
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]	-	-	130000	-	0.082
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Citations

View all 10 citations: Citation Explorer

Inhibiting fibronectin polymerization alleviates kidney injury due to ischemia/reperfusion
Authors: Bowers, Stephanie LK and Davis-Rodriguez, Stephanie and Thomas, Zachary M and Rudomanova, Valeriia and Bacon, W Clark and Beiersdorfer, Alex and Ma, Qing and Devarajan, Prasad and Blaxall, Burns C
Journal: American Journal of Physiology-Renal Physiology (2019)

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16

References

View all 25 references: Citation Explorer

Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Page updated on December 17, 2024

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Catalog Number	2281
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Physical properties

Molecular weight	1050.35
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.027
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	649
Emission (nm)	663
Quantum yield	0.27¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501