Tide Fluor™ 5WS azide [TF5WS azide]

Tide Fluor™ 5WS (TF5WS) family has the spectral properties essentially identical to those of Cy5. Compared to Cy5 probes TF5WS family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy5. TF5WS-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy5. In pairing with our Tide Quencher™ 5WS (TQ5WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF5WS product is reactive to alkynes, and useful for click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 5WS azide [TF5WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.051 µL	480.257 µL	960.513 µL	4.803 mL	9.605 mL
5 mM	19.21 µL	96.051 µL	192.103 µL	960.513 µL	1.921 mL
10 mM	9.605 µL	48.026 µL	96.051 µL	480.257 µL	960.513 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
Tide Fluor™ 1 azide [TF1 azide]	341	447	20000	0.91¹	0.187
Tide Fluor™ 2 azide [TF2 azide]	503	525	75000	0.9¹	0.09
Tide Fluor™ 3 azide [TF3 azide]	553	578	75000¹	0.1¹	0.179
Tide Fluor™ 5WS acid [TF5WS acid] Superior replacement for Cy5	649	663	250000	0.27¹	0.027
Tide Fluor™ 5WS amine [TF5WS amine] Superior replacement for Cy5	649	663	250000	0.27¹	0.027
Tide Fluor™ 5WS maleimide [TF5WS maleimide] Superior replacement for Cy5	649	663	250000	0.27¹	0.027
Tide Fluor™ 4 azide [TF4 azide]	577	602	90000	0.77¹	0.436
Tide Fluor™ 6WS azide [TF6WS azide]	682	701	220000	0.27¹	0.101
Tide Fluor™ 7WS azide [TF7WS azide]	756	780	275000	0.3¹	0.049
Tide Fluor™ 8WS azide [TF8WS azide] Near Infrared Emission	785	801	250000	0.21¹	0.109
Tide Quencher™ 5WS azide [TQ5WS azide]	-	-	130000	-	0.082
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Citations

View all 7 citations: Citation Explorer

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16

Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Page updated on November 21, 2024

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Catalog Number	2275
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Physical properties

Molecular weight	1041.11
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.027
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	649
Emission (nm)	663
Quantum yield	0.27¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501