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Telephone:1-408-733-1055
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Pleasanton, CA, 94588
USA

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United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

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Tide Fluor™ 1 CPG [TF1 CPG] *1000 Å* *Superior replacement for EDANS*

TF1 is designed to be a superior fluorophore alternative to EDANS. TF1 has (a). much stronger absorption; (b). much stronger fluorescence intensity; and (3). much less environment-sensitive fluorescence. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to EDANS. TF1-labeled peptides and nucleotides exhibit much stronger fluorescence and higher sensitivity than the ones labeled with EDANS. In pairing with our Tide Quencher™ 1 (TQ1), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
Tide Fluor™ 1 azide [TF1 azide]341447200000.9110.187
Tide Fluor™ 1 alkyne [TF1 alkyne]341447200000.9110.187
Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS*341447200000.9110.187
Tide Fluor™ 1 amine [TF1 amine] *Superior replacement for EDANS*341447200000.9110.187
Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS*341447200000.9110.187
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*--20000-0.194
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*--20000-0.194

Citations

View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Page updated on November 21, 2024

Ordering information

Price
Unit size
Catalog Number2241
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Solvent

MeCN

Spectral properties

Correction Factor (280 nm)

0.187

Extinction coefficient (cm -1 M -1)

20000

Excitation (nm)

341

Emission (nm)

447

Quantum yield

0.911

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
<p style="background: white; margin: 6.0pt 0in 6.0pt 0in;"><span style="font-size: 10.0pt;">Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled. Each addition is referred to as a synthetic cycle and consists of four chemical reactions: d<span class="mw-headline">e-blocking (detritylation)</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, coupling</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, capping</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, and oxidation</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">.</span></span></p>
<p style="background: white; margin: 6.0pt 0in 6.0pt 0in;"><span style="font-size: 10.0pt;">Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled. Each addition is referred to as a synthetic cycle and consists of four chemical reactions: d<span class="mw-headline">e-blocking (detritylation)</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, coupling</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, capping</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, and oxidation</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">.</span></span></p>
<p style="background: white; margin: 6.0pt 0in 6.0pt 0in;"><span style="font-size: 10.0pt;">Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled. Each addition is referred to as a synthetic cycle and consists of four chemical reactions: d<span class="mw-headline">e-blocking (detritylation)</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, coupling</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, capping</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">, and oxidation</span><span style="user-select: none; display: inline-block; unicode-bidi: isolate;">.</span></span></p>

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Peptide and Oligonucleotide Labeling
Digital PCR
Polymerase Chain Reaction (PCR)
Real-Time PCR (qPCR)
Reverse Transcription PCR (RT-PCR)
RNA Purification & Analysis
Tide Fluor Dyes

Alternative formats

Tide Fluor™ 1 CPG [TF1 CPG] *500 Å* *Superior replacement for EDANS*
Tide Fluor™ 1 succinimidyl ester [TF1 SE]*Superior replacement for EDANS*
Tide Fluor™ 2 acid [TF2 acid] *Superior replacement for fluorescein*
Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein*