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Thiolite™ Green

Thiolite™ Green is one of the most sensitive sensors for measuring thiol compounds. It gives a green fluorescent adduct upon reacting with thiol compounds (such as cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Thiolite™ Blue335460

Citations

View all 8 citations: Citation Explorer
Carboranyl-1, 8-naphthalimide intercalators induce lysosomal membrane permeabilization and ferroptosis in cancer cell lines
Authors: Rykowski, Sebastian and Gurda-Wo{\'z}na, Dorota and Fedoruk-Wyszomirska, Agnieszka and Orlicka-P{\l}ocka, Marta and Kowalczyk, Aleksandra and St{\k{a}}czek, Pawe{\l} and Denel-Bobrowska, Marta and Biniek-Antosiak, Katarzyna and Rypniewski, Wojciech and Wyszko, Eliza and others,
Journal: Journal of Enzyme Inhibition and Medicinal Chemistry (2023): 2171028
Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives
Authors: Orlicka-P{\l}ocka, Marta and Fedoruk-Wyszomirska, Agnieszka and Gurda-Wo{\'z}na, Dorota and Pawelczak, Pawe{\l} and Krawczyk, Patrycja and Giel-Pietraszuk, Ma{\l}gorzata and Framski, Grzegorz and Ostrowski, Tomasz and Wyszko, Eliza
Journal: Antioxidants (2021): 950
Revealing 3D structure of gluten in wheat dough by optical clearing imaging
Authors: Ogawa, Takenobu and Matsumura, Yasuki
Journal: Nature communications (2021): 1--10
Circumventing the Crabtree effect: forcing oxidative phosphorylation (OXPHOS) via galactose medium increases sensitivity of HepG2 cells to the purine derivative kinetin riboside
Authors: Orlicka-P{\l}ocka, Marta and Gurda-Wozna, Dorota and Fedoruk-Wyszomirska, Agnieszka and Wyszko, Eliza
Journal: Apoptosis (2020): 835--852
Quercetin Triggers Induction of Apoptotic and Lysosomal Death of Sensitive and Multidrug Resistant Leukaemia HL60 Cells
Authors: Maruszewska, Agnieszka and Tarasiuk, Jolanta
Journal: Nutrition and Cancer (2020): 1--18

References

View all 74 references: Citation Explorer
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194
Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)
A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132
Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier
Authors: Hu B, Zhao Y, Zhu HZ, Yu SH.
Journal: ACS Nano (2011): 3166
Simple and rapid determination of thiol compounds by HPLC and fluorescence detection with 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide) difluoroboradiaza-s-indacene
Authors: Guo XF, Zhao PX, Wang H, Zhang HS.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2011): 3932
Page updated on November 21, 2024

Ordering information

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Unit size
Catalog Number21508
Quantity
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Physical properties

Molecular weight

~400

Solvent

DMSO

Spectral properties

Excitation (nm)

505

Emission (nm)

524

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Spectrum for Thiolite Green
Spectrum for Thiolite Green
Spectrum for Thiolite Green

Alternative formats