Thioflavin T

Thioflavin T (ThT), also called Basic Yellow 1 or CI 49005, is a benzothiazole salt. The dye is used to visualize plaques composed of beta-amyloid found in the brains of Alzheimer's disease patients. When it binds to beta sheets, such as those in amyloid oligomers, the dye undergoes a characteristic 120 nm red shift of its excitation spectrum that may be selectively excited at 450 nm, resulting in a fluorescence signal at 482 nm. ThT binds rapidly and specifically to the anti-parallel beta-sheet fibrils formed from synthetic beta-amyloid (1-40), but does not bind to monomer or oligomeric intermediates. The binding of ThT does not interfere with the aggregation of beta-amyloid peptide into amyloid fibrils. Thioflavin T acid is a valuble tool for making various bioconjugates of Thioflavin T that can direct Thioflavin T to a desired target. It is an essential reagent to make the peptides and other conjugates of Thioflavin T.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Thioflavin T acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.39 µL	1.007 mL	2.014 mL	10.069 mL	20.139 mL
5 mM	40.278 µL	201.39 µL	402.779 µL	2.014 mL	4.028 mL
10 mM	20.139 µL	100.695 µL	201.39 µL	1.007 mL	2.014 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Citations

View all 9 citations: Citation Explorer

Tau seeding in a HEK cell model is influenced by aggregation inducers and cellular chaperones
Authors: McNamara, Emily
Journal: (2022)

Human Telomeric Hybrid-2-over-Hybrid-1 G-quadruplex Targeting and a Selective Hypersaline-Tolerant Sensor Using Abasic Site-Engineered Monomorphism
Authors: Wu, Tao and Ye, Meiyun and Mao, Tianyi and Lin, Fan and Hu, Yuehua and Gan, Ning and Shao, Yong
Journal: Analytica Chimica Acta (2017)

Fluorescent dyes in the context of DNA-binding: The case of Thioflavin T
Authors: Biancardi, Aless and ro , undefined and Biver, Tarita and Mennucci, Benedetta
Journal: International Journal of Quantum Chemistry (2017)

Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels
Authors: Sugimoto, Shinya and Arita-Morioka, Ken-ichi and Mizunoe, Yoshimitsu and Yamanaka, Kunitoshi and Ogura, Teru
Journal: Nucleic acids research (2015): gkv338

Determinants of amyloid fibril degradation by the PDZ protease HTRA1
Authors: Poepsel, Simon and Sprengel, Andreas and Sacca, Barbara and Kaschani, Farnusch and Kaiser, Markus and Gatsogiannis, Christos and Raunser, Stefan and Clausen, Tim and Ehrmann, Michael
Journal: Nature chemical biology (2015): 862--869

References

View all 28 references: Citation Explorer

Methods for enhancing the accuracy and reproducibility of Congo red and thioflavin T assays
Authors: Eisert R, Felau L, Brown LR.
Journal: Anal Biochem (2006): 144

Lipophilic analogs of thioflavin S as novel amyloid-imaging agents
Authors: Wu C, Cai L, Wei J, Pike VW, Wang Y.
Journal: Curr Alzheimer Res (2006): 259

Oligo(ethylene glycol) derivatives of Thioflavin T as inhibitors of protein-amyloid interactions
Authors: Inbar P, Li CQ, Takayama SA, Bautista MR, Yang J.
Journal: Chembiochem (2006): 1563

Mechanism of thioflavin T accumulation inside cells overexpressing P-glycoprotein or multidrug resistance-associated protein: role of lipophilicity and positive charge
Authors: Darghal N, Garnier-Suillerot A, Salerno M.
Journal: Biochem Biophys Res Commun (2006): 623

Characteristics of the binding of thioflavin S to tau paired helical filaments
Authors: Santa-Maria I, Perez M, Hern and ez F, Avila J, Moreno FJ.
Journal: J Alzheimers Dis (2006): 279

Page updated on October 10, 2024

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Physical properties

Molecular weight	496.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	199
Excitation (nm)	349
Emission (nm)	454

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200