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Texas Red-DHPE *CAS 187099-99-6*

Texas Red® DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent Texas Red® dye (excitation/emission maxima ~595/615 nm). Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. Texas Red DHPE is principally employed as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the Texas Red dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy. This technique has enabled visualization of ATP-dependent fusion of liposomes with the Golgi apparatus. Membrane flux during hemagglutinin-mediated cell-cell fusion has been visualized using Texas Red DHPE and the lipophilic carbocyanine DiI as membrane labels.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-DHPE *CAS 187099-99-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM72.367 µL361.836 µL723.673 µL3.618 mL7.237 mL
5 mM14.473 µL72.367 µL144.735 µL723.673 µL1.447 mL
10 mM7.237 µL36.184 µL72.367 µL361.836 µL723.673 µL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (565 nm)
Texas Red® hydrazide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® cadaverine *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® maleimide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® azide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® alkyne *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red TCO *Single isomer*58660311600010.971, 0.9320.230.360.4472

Citations

View all 5 citations: Citation Explorer
A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis
Authors: Ashby, Grant and Keng, Kayla E and Hayden, Carl C and Stachowiak, Jeanne
Journal: bioRxiv (2024): 2024--02
Interfacial Viscoelasticity and Structure of Self-Assembled Semifluorinated Alkanes on Water
Authors: Mielke, Salom{\'e}
Journal: (2019)
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Presenting Author (s)
Authors: Peshin, Snehan and Yao, Ming and Wang, Yuli and Allbritton, Nancy L

References

View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Page updated on December 17, 2024

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Physical properties

Molecular weight

1381.84

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm -1 M -1)

1160001

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.971, 0.932

Storage, safety and handling

H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

187099-99-6