Tetracysteine FLASH diacetate *2 mM DMSO Solution*

Tetracysteine FLASH diacetate

2 mM DMSO Solution

FLASH diacetate is a cell-permeable version of FlASH, which is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other. It was developed by Roger Tsien and colleagues in 1998. The biarsenical labeling technology works through the high-affinity interaction of arsenic for thiols. When FlASH binds to tetracysteine (TC) sequences, its biarsenical group reacts rapidly with Cys-Cys moiety and the tag becomes highly fluorescent in green. The biarsenical labeling reagent FLASH is the smallest expression tag for labeling a protein that contains a six-amino acid motif with a Cys-Cys-X1-X2-Cys-Cys amino acid sequence. The most commonly used tetracysteine is the six amino acid Cys-Cys-Pro-Gly-Cys-Cys sequence. As this sequence rarely appears in endogenous proteins, incorporating the sequence into target proteins generates a small but highly specific target for protein labeling. FLASH generates a strong green fluorescent signal when binding to recombinant proteins containing the tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys. It can be used for monitoring protein localization, turnover and trafficking, receptor signaling and internalization.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22334	100 Tests	Price

References

View all 24 references: Citation Explorer

Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.

Authors:

Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert

Journal:

PloS one (2019): e0210704

Probing Arrestin Function Using Intramolecular FlAsH-BRET Biosensors.

Authors:

Strungs, Erik G and Luttrell, Louis M and Lee, Mi-Hye

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 309-322

Probing the Effect of Sildenafil on Progesterone and Testosterone Production by an Intracellular FRET/BRET Combined Approach.

Authors:

Casarini, Livio and Riccetti, Laura and Limoncella, Silvia and Lazzaretti, Clara and Barbagallo, Federica and Pacifico, Salvatore and Guerrini, Remo and Tagliavini, Simonetta and Trenti, Tommaso and Simoni, Manuela and Sola, Marco and Di Rocco, Giulia

Journal:

Biochemistry (2019): 799-808

Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.

Authors:

Ng, Janice S W and Hanspal, Maya A and Matharu, Naunehal S and Barros, Teresa P and Esbjörner, Elin K and Wilson, Mark R and Yerbury, Justin J and Dobson, Christopher M and Kumita, Janet R

Journal:

Biochemistry (2019): 4086-4095

The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labeling.

Authors:

Tsai, Chuan-Fu and Lin, Hsin-Yi and Hsu, Wei-Li and Tsai, Ching-Hsiu

Journal:

FEBS open bio (2017): 1960-1971

Physical properties

Molecular weight	748.56
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 17, 2025