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TET-dT Phosphoramidite
TET-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the TET tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-TET phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, HEX-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 | 0.13 |
| HEX-dT Phosphoramidite | 533 | 559 | 73000 | 0.15 |
| Fluorescein-dT Phosphoramidite | 498 | 517 | 800001 | 0.35 |
References
View all 1 references: Citation Explorer
Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.
Authors: Riml, Christian and Micura, Ronald
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 295-302
Authors: Riml, Christian and Micura, Ronald
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 295-302
Page updated on November 20, 2024
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