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Sulforhodamine 101, sodium salt
Sulforhodamine 101 is a fluorescence reference standard for measuring fluorescence quantum yield. It is also the key precursor used for preparing rhodamine 101 sulfonyl chloride (also called Texas Red®, a trademark of Molecular Probes). This sulforhodamine 101 sodium salt is more water-soluble than the neutral sulforhodamine 101.
Example protocol
AT A GLANCE
Important
Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. Sulforhodamine 101 stock solution (1mM):
Add 1.591 µL DMSO or water to 1 mg Sulforhodamine 101 to make 1 mM stock solution. Note: Add appropriate amount of DMSO or water to make appropriate concentration of stock solution.
PREPARATION OF WORKING SOLUTION
1. Sulforhodamine 101 working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7-8. Note: Make appropriate concentration of working solution as per needed.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine 101, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 159.061 µL | 795.305 µL | 1.591 mL | 7.953 mL | 15.906 mL |
| 5 mM | 31.812 µL | 159.061 µL | 318.122 µL | 1.591 mL | 3.181 mL |
| 10 mM | 15.906 µL | 79.53 µL | 159.061 µL | 795.305 µL | 1.591 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
References
View all 4 references: Citation Explorer
Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Flow cytometric applications of Sulforhodamine 101 as a fluorescent stain for total cellular protein
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1
Authors: Engelhard HH., undefined
Journal: Biotech Histochem (1997): 1
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482
Page updated on December 17, 2024
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