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Sulfide Green™ AM

Sulfide Green™ AM is a fluorogenic probe for detecting hydrogen sulfide in live cells. It is cell-permeable for use in live cell for imaging sulfide anion or hydrogen sulfide Sulfide Green™ AM contains the hydrophobic acetoxymethyl ester groups, making it cell-permeable. When Sulfide Green™ AM gets in live cells, its AM moieties are cleaved by esterases. The esterase-induced hydrolysis of Sulfide Green™ AM generates a negatively charged fluorogenic product that is well retained in cells. Upon reaction with sulfide or hydrogen sulfide, Sulfide Green generates strong green fluorescence that is proportional to sulfide concentration. The activation of Sulfide Green™ AM has been shown to detect as low as nM sulfide anion in cells.

Spectrum

Citations

View all 29 citations: Citation Explorer
Haloalkaliphilic microorganisms assist sulfide removal in a microbial electrolysis cell
Authors: Ni, G., Harnawan, P., Seidel, L., Ter Heijne, A., Sleutels, T., Buisman, C. J. N., Dopson, M.
Journal: J Hazard Mater (2019): 197-204
Pharmacological levels of hydrogen sulfide inhibit oxidative cell injury through regulating the redox state of thioredoxin
Authors: Mao, Z., Huang, Y., Zhang, Z., Yang, X., Zhang, X., Huang, Y., Sawada, N., Mitsui, T., Takeda, M., Yao, J.
Journal: Free Radic Biol Med (2019): 190-199
Sulfide-mediated azo dye degradation and microbial community analysis in a single-chamber air cathode microbial fuel cell
Authors: Dai, Q., Zhang, S., Liu, H., Huang, J., Li, L.
Journal: Bioelectrochemistry (2019): 107349
Corrigendum to "Synergistic growth inhibition of mouse skin tumors by pomegranate fruit extract and diallyl sulfide: Evidence for inhibition of activated MAPKs/NF-kappaB and reduced cell proliferation" [Food Chem. Toxicol. 49 (2011) 1511-20]
Authors: George, J., Singh, M., Srivastava, A. K., Bhui, K., Shukla, Y.
Journal: Food Chem Toxicol (2019): 110784
Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria
Authors: Sahebekhtiari, N., Fern and ez-Guerra, P., Nochi, Z., Carlsen, J., Bross, P., Palmfeldt, J.
Journal: Biochim Biophys Acta Mol Basis Dis (2019): 126-135
Page updated on November 21, 2024

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Catalog Number21301
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Physical properties

Molecular weight

685.56

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

500

Emission (nm)

522

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure

CAS

N/A
Na2S dose response was measured with Sulfide Green&trade; AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 &micro;M. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.
Na2S dose response was measured with Sulfide Green&trade; AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 &micro;M. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.
Na2S dose response was measured with Sulfide Green&trade; AM. Na2S standards serially diluted in 20mM HEPES buffer were added to each well to reach the final concentration of 10 &micro;M. The assay was incubated at room temperature for 60 min, and then measure Ex/Em= 490/530nm, cutoff=515nm.