Stayright™ Purple HRP Staining Kit
Example protocol
AT A GLANCE
Apply the Stayright™ Purple working solution to the tissue section.
Incubate the tissue section for 5 to 15 minutes.
Rinse the tissue sections for 5 to 10 minutes, then if needed proceed with counterstaining.
Apply the mounting medium to cover the section.
PREPARATION OF WORKING SOLUTION
Prepare a Stayright™ Purple working solution by adding 10 μL of 100X Stayright™ Purple (Component A) and 1 μL of stabilized 3% Hydrogen peroxide (Component C) to every 1 mL of Stayright™ Purple HRP buffer (Component B).
Note: Unused pre-mixed working solution can be stored at 2-4°C for a few weeks. However, we recommend preparing the mixture fresh for each application.
SAMPLE EXPERIMENTAL PROTOCOL
Apply the Stayright™ Purple working solution to the tissue section and let it incubate at room temperature for 5 to 15 minutes.
Immerse the slides in dH2O to stop the color development process. Monitor the staining intensity carefully. If the staining intensity is insufficient, a longer incubation period is needed. You can reapply the Stayright™ Purple working solution to further develop the staining.
Wash the tissue sections with dH2O for 5 to 10 minutes.
Use a desired counterstain if necessary.
Dehydrate the specimen using ethanol and then mount it permanently in an organic mounting medium.
Note: For guidelines on sample preparation, please visit
https://www.aatbio.com/resources/guides/paraffin-embedded-tissue-immunohistochemistry-protocol.html
Citations
Authors: Takashima, Tsuyoshi and Zeng, Chao and Murakami, Eitaro and Fujiwara, Naoko and Kohara, Masaharu and Nagata, Hideki and Feng, Zhaozu and Sugai, Ayako and Harada, Yasue and Ichijo, Rika and others,
Journal: JCI Insight (2025)
Authors: Kashiwagi, Mitsuaki and Beck, Goichi and Kanuka, Mika and Arai, Yoshifumi and Tanaka, Kaeko and Tatsuzawa, Chika and Koga, Yumiko and Saito, Yuki C and Takagi, Marina and Oishi, Yo and others,
Journal: Cell (2024)
Authors: Zhang, S., Yang, J., Lin, J.
Journal: Bioelectrochemistry (2008): 47-52
Authors: Thomas, M. A., Lemmer, B.
Journal: Brain Res Brain Res Protoc (2005): 107-18
Authors: Kiernan, J. A.
Journal: Biotech Histochem (2003): 135