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ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

ROX Reference Dye is equivalent to 12223-012 (Life Technology). It provides users of the ABI PRISM® 7700 a convenient method for normalizing fluorescent reporter signal in real-time quantitative PCR or RT-PCR without modifying the instrument's default analysis parameters. ROX Reference Dye is supplied at 50X concentration. ROX Reference Dye is made of highly purified 5-ROX glycine. Compared to the standard ROX dyes 5-ROX glycine has improved stability.

Spectrum

References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on October 30, 2024

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Catalog Number400
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Physical properties

Molecular weight

N/A

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.168

Extinction coefficient (cm -1 M -1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.941

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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