Rhodamine B CAS 81-88-9

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	479.01
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

81-88-9

Molecular weight

479.01

Absorbance (nm)

541

Extinction coefficient (cm ^-1 M ^-1)

106000¹

Excitation (nm)

546

Emission (nm)

567

Quantum yield

0.7¹, 0.43²

Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.764 µL	1.044 mL	2.088 mL	10.438 mL	20.876 mL
5 mM	41.753 µL	208.764 µL	417.528 µL	2.088 mL	4.175 mL
10 mM	20.876 µL	104.382 µL	208.764 µL	1.044 mL	2.088 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Rhodamine 800 CAS 137993-41-0	682	704	-
Rhodamine 6G CAS 989-38-8	525	548	116000¹
Sulforhodamine B CAS 3520-42-1	559	577	-
Rhodamine 110 CAS 13558-31-1	500	522	80000
Rhodamine 123 CAS 62669-70-9	508	528	85200
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000

Images

Figure 1. Chemical structure for Rhodamine B *CAS 81-88-9*

Citations

View all 1 citations: Citation Explorer

Optothermal crystallization of hard spheres in an effective bidimensional geometry
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)

References

View all 39 references: Citation Explorer

Adsorption behavior of rhodamine B on Rhizopus oryzae biomass
Authors: Das SK, Bhowal J, Das AR, Guha AK.
Journal: Langmuir (2006): 7265

Controllable size, shape and morphology of molybdic acid self-aggregated with rhodamine B to construct functional material
Authors: Guan ZS, Zhang Y, Zhang Q, Li DX.
Journal: J Colloid Interface Sci (2006): 113

Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009

Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779

Fluorescent plasma nanocomposite thin films containing nonaggregated rhodamine 6G laser dye molecules
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719

Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306

Resonance scattering effect of rhodamine dye association nanoparticles and its application to respective determination of trace ClO2 and Cl2
Authors: Jiang ZL, Zhou SM, Liang AH, Kang C, He X.
Journal: Environ Sci Technol (2006): 4286

Complex formation between rhodamine B and poly(sodium 4-styrenesulfonate) studied by 1H-NMR
Authors: Moreno-Villoslada I, Gonzalez R, Hess S, Rivas BL, Shibue T, Nishide H.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 21576

Ab initio study of optical properties of rhodamine 6G molecular dimers
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301

Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)