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RH 421 [N-(4-Sulfobutyl)-4-(4-(4- (dipentylamino)phenyl)butadienyl)pyridinium, inner salt] *CAS 107610-19-5*
RH 421, also called N-(4-Sulfobutyl)-4-(4-(4- (dipentylamino)phenyl)butadienyl)pyridinium, is a neuron-tracing dye. It is used for monitoring membrane potential, synaptic activity and ion channel activity of neurons.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of RH 421 [N-(4-Sulfobutyl)-4-(4-(4- (dipentylamino)phenyl)butadienyl)pyridinium, inner salt] *CAS 107610-19-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 200.513 µL | 1.003 mL | 2.005 mL | 10.026 mL | 20.051 mL |
| 5 mM | 40.103 µL | 200.513 µL | 401.027 µL | 2.005 mL | 4.01 mL |
| 10 mM | 20.051 µL | 100.257 µL | 200.513 µL | 1.003 mL | 2.005 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) |
| RH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt] | 550 | 786 |
| RH 795 *CAS#: 172807-13-5* | 486 | 689 |
References
View all 27 references: Citation Explorer
Palytoxin-induced effects on partial reactions of the Na,K-ATPase
Authors: Harmel N, Apell HJ.
Journal: J Gen Physiol (2006): 103
Authors: Harmel N, Apell HJ.
Journal: J Gen Physiol (2006): 103
Electrogenic partial reactions of the gastric H,K-ATPase
Authors: Diller A, Vagin O, Sachs G, Apell HJ.
Journal: Biophys J (2005): 3348
Authors: Diller A, Vagin O, Sachs G, Apell HJ.
Journal: Biophys J (2005): 3348
Effective gating charge of ion channels induced by toxin syringomycin E in lipid bilayers
Authors: Schagina LV, Gurnev PA, Takemoto JY, Malev VV.
Journal: Bioelectrochemistry (2003): 21
Authors: Schagina LV, Gurnev PA, Takemoto JY, Malev VV.
Journal: Bioelectrochemistry (2003): 21
Effect of dipole modifiers on the kinetics of sensitized photoinactivation of gramicidin channels in bilayer lipid membranes
Authors: Antonenko YN, Rokitskaya TI, Kotova EA.
Journal: Membr Cell Biol (1999): 111
Authors: Antonenko YN, Rokitskaya TI, Kotova EA.
Journal: Membr Cell Biol (1999): 111
Hofmeister effects of anions on the kinetics of partial reactions of the Na+,K+-ATPase
Authors: Ganea C, Babes A, Lupfert C, Grell E, Fendler K, Clarke RJ.
Journal: Biophys J (1999): 267
Authors: Ganea C, Babes A, Lupfert C, Grell E, Fendler K, Clarke RJ.
Journal: Biophys J (1999): 267
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