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Resorufin, sodium salt *CAS 34994-50-8*

Resorufin is used as a calibration standard for resorufin-derived enzyme substrates. It has pH dependent absorption and emission over the range of 5 to 9. It is also the reduction product of resazurin in cells.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*33645559000.107
Resazurin, sodium salt *CAS 62758-13-8*571584--

References

View all 31 references: Citation Explorer
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Induction of ethoxy-resorufin-O-deethylase activity by halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons in primary hepatocytes of the green frog (Rana esculenta)
Authors: Rankouhi TR, Koomen B, S and erson JT, Bosveld AT, Seinen W, van den Berg M.
Journal: Environ Toxicol Chem (2005): 1428
Validation and diagnostic efficacy of a lipase assay using the substrate 1,2-o-dilauryl-rac-glycero glutaric acid-(6' methyl resorufin)-ester for the diagnosis of acute pancreatitis in dogs
Authors: Graca R, Messick J, McCullough S, Barger A, Hoffmann W.
Journal: Vet Clin Pathol (2005): 39
Quantitative structure-activity relationships within a homologous series of 7-alkoxyresorufins exhibiting activity towards CYP1A and CYP2B enzymes: molecular modelling studies on key members of the resorufin series with CYP2C5-derived models of human CYP1A
Authors: Lewis DF, Lake BG, Dickins M.
Journal: Xenobiotica (2004): 501
A resorufin-based fluorescent assay for quantifying NADH
Authors: Batchelor RH, Zhou M.
Journal: Anal Biochem (2002): 118
Page updated on October 28, 2024

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Physical properties

Molecular weight

235.17

Solvent

Water

Spectral properties

Absorbance (nm)

570

Extinction coefficient (cm -1 M -1)

650001

Excitation (nm)

571

Emission (nm)

584

Quantum yield

0.751

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

34994-50-8
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