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Resorufin Phosphocholine
Glycerophosphocholine phosphodiesterase release phosphocholine from resorufin phosphocholine to give the highly fluorescent resorufin that can be readily monitored by either fluorescence or absorption. This fluorogenic phosphodiesterase substrate can be conveniently used for screening the inhibitors of glycerophosphocholine phosphodiesterase. It can also be used for monitoring the activities of phospholipase D in combination with a phosphatase. Phospholipase D hydrolyzes resorufin phosphocholine to resorufin phosphate, which in the presence of an acid phosphatase generates resorufin. Thus, resorufin phosphocholine can also be used for monitoring phospholipase D in sources that do not also contain high levels of phospholipase C.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Resorufin Phosphocholine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 264.326 µL | 1.322 mL | 2.643 mL | 13.216 mL | 26.433 mL |
| 5 mM | 52.865 µL | 264.326 µL | 528.653 µL | 2.643 mL | 5.287 mL |
| 10 mM | 26.433 µL | 132.163 µL | 264.326 µL | 1.322 mL | 2.643 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 81 references: Citation Explorer
Identification of phosphotyrosine mimetic inhibitors of human tyrosyl-DNA phosphodiesterase I by a novel AlphaScreen high-throughput assay
Authors: March, undefined and C, Lea WA, Jadhav A, Dexheimer TS, Austin CP, Inglese J, Pommier Y, Simeonov A.
Journal: Mol Cancer Ther (2009): 240
Authors: March, undefined and C, Lea WA, Jadhav A, Dexheimer TS, Austin CP, Inglese J, Pommier Y, Simeonov A.
Journal: Mol Cancer Ther (2009): 240
The effects of a type 4 phosphodiesterase inhibitor and the muscarinic cholinergic antagonist tolterodine tartrate on detrusor overactivity in female rats with bladder outlet obstruction
Authors: Kaiho Y, Nishiguchi J, Kwon DD, Chancellor MB, Arai Y, Snyder PB, Yoshimura N.
Journal: BJU Int (2008): 615
Authors: Kaiho Y, Nishiguchi J, Kwon DD, Chancellor MB, Arai Y, Snyder PB, Yoshimura N.
Journal: BJU Int (2008): 615
Antiplatelet cilostazol, an inhibitor of type III phosphodiesterase, improves swallowing function in patients with a history of stroke
Authors: Teramoto S, Yamamoto H, Yamaguchi Y, Ishii M, Hibi S, Kume H, Ouchi Y.
Journal: J Am Geriatr Soc (2008): 1153
Authors: Teramoto S, Yamamoto H, Yamaguchi Y, Ishii M, Hibi S, Kume H, Ouchi Y.
Journal: J Am Geriatr Soc (2008): 1153
Inhibition of platelet aggregation by olive oil phenols via cAMP-phosphodiesterase
Authors: Dell'Agli M, Maschi O, Galli GV, Fagnani R, Dal Cero E, Caruso D, Bosisio E.
Journal: Br J Nutr (2008): 945
Authors: Dell'Agli M, Maschi O, Galli GV, Fagnani R, Dal Cero E, Caruso D, Bosisio E.
Journal: Br J Nutr (2008): 945
Lipolytic effect of a polyphenolic citrus dry extract of red orange, grapefruit, orange (SINETROL) in human body fat adipocytes. Mechanism of action by inhibition of cAMP-phosphodiesterase (PDE)
Authors: Dallas C, Gerbi A, Tenca G, Juchaux F, Bernard FX.
Journal: Phytomedicine (2008): 783
Authors: Dallas C, Gerbi A, Tenca G, Juchaux F, Bernard FX.
Journal: Phytomedicine (2008): 783
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