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Resorufin methyl ether *CAS 5725-89-3*
This fluorogenic resorufin ether is widely used for monitoring P450 activities in cell extracts and solutions. Cytochrome P450 oxidase (CYP450) is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin methyl ether *CAS 5725-89-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 440.121 µL | 2.201 mL | 4.401 mL | 22.006 mL | 44.012 mL |
| 5 mM | 88.024 µL | 440.121 µL | 880.243 µL | 4.401 mL | 8.802 mL |
| 10 mM | 44.012 µL | 220.061 µL | 440.121 µL | 2.201 mL | 4.401 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin benzyl ether *CAS 87687-02-3* | 571 | 584 | 650001 | 0.751 |
| Resorufin ethyl ether *CAS 5725-91-7* | 571 | 584 | 650001 | 0.751 |
| Resorufin pentyl ether *CAS 87687-03-4* | 571 | 584 | 650001 | 0.751 |
Citations
View all 1 citations: Citation Explorer
Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tröbs, S and Knöspel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tröbs, S and Knöspel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290
References
View all 57 references: Citation Explorer
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Comparison of cytochrome P450 inhibition assays for drug discovery using human liver microsomes with LC-MS, rhCYP450 isozymes with fluorescence, and double cocktail with LC-MS
Authors: Di L, Kerns EH, Li SQ, Carter GT.
Journal: Int J Pharm. (2006)
Authors: Di L, Kerns EH, Li SQ, Carter GT.
Journal: Int J Pharm. (2006)
Ginkgo biloba does not alter clearance of flurbiprofen, a cytochrome P450-2C9 substrate
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Cytochrome P450 (CYP) inhibition screening: comparison of three tests
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
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