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Resorufin beta-D-galactopyranoside *CAS 95079-19-9*
Resorufin beta-D-galactopyranoside is a fluorogenic galactosidase substrate that generates a red fluorescent product upon interaction with the enzyme. This enzyme substrate is widely used in high throughput screening of galactosidase inhibitors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resorufin beta-D-galactopyranoside *CAS 95079-19-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 266.432 µL | 1.332 mL | 2.664 mL | 13.322 mL | 26.643 mL |
| 5 mM | 53.286 µL | 266.432 µL | 532.864 µL | 2.664 mL | 5.329 mL |
| 10 mM | 26.643 µL | 133.216 µL | 266.432 µL | 1.332 mL | 2.664 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin alpha-D-galactopyranoside | 571 | 584 | 650001 | 0.751 |
References
View all 8 references: Citation Explorer
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Authors: English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.
Journal: Nat Chem Biol (2006): 87
Authors: English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS.
Journal: Nat Chem Biol (2006): 87
Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules
Authors: Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
Journal: Biochemistry (2003): 1707
Authors: Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
Journal: Biochemistry (2003): 1707
Measurement of enzyme kinetics using a continuous-flow microfluidic system
Authors: Seong GH, Heo J, Crooks RM.
Journal: Anal Chem (2003): 3161
Authors: Seong GH, Heo J, Crooks RM.
Journal: Anal Chem (2003): 3161
beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate
Authors: Eggertson MJ, Craig DB.
Journal: Biomed Chromatogr (1999): 516
Authors: Eggertson MJ, Craig DB.
Journal: Biomed Chromatogr (1999): 516
Microchip device for performing enzyme assays
Authors: Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM.
Journal: Anal Chem (1997): 3407
Authors: Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM.
Journal: Anal Chem (1997): 3407
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